Related Experiment Video
Updated: Jul 11, 2026

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
Published on: October 19, 2010
Immunofluorescence technique for the detection of salmonellae in various foods
N F Insalata1, S J Schulte, J H Berman
1General Foods Technical Center, Tarrytown, New York 10591.
Abstract:
Salmonella species have been detected in nine food varieties by use of fluorescent antibodies without false-positive or false-negative results. Test antisera were specially prepared, commercially available, conjugated polyvalent O globulin absorbed with cultures of Escherichia coli and Citrobacter freundii, and polyvalent phase II H globulin antibodies. Use of this technique permits a decrease of 24 hr in time normally required for Salmonella detection when compared with cultural Salmonella recovery methods.
Related Concept Videos
FISH - Fluorescent In-situ Hybridization
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Immunofluorescence Microscopy
The...
Special Staining Techniques

