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Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry
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Improved cell dispersal method by low-temperature incubation of tissue in a trypsin-citrate solution

A L van Wezel1, P A van Hemert, W Parisius

  • 1Rijks Instituut voor de Volksgezondheid, Postbus 1, Bilthoven, The Netherlands.

Applied Microbiology
|September 1, 1972
PubMed

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Tissue Homogenization and Cell Lysis01:32

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Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...