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A High Throughput Screen for Biomining Cellulase Activity from Metagenomic Libraries
Published on: February 1, 2011
Cellulase Production by Trichoderma viride on Feedlot Waste
H L Griffin1, J H Sloneker, G E Inglett
1Northern Regional Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604.
Applied Microbiology
|June 1, 1974
Summary
Batch fermentation of feedlot waste using Trichoderma viride efficiently utilizes carbohydrates and produces valuable cellulase enzymes. The resulting fermented waste is nearly odor-free, retaining nitrogen while reducing organic matter.
Area of Science:
- Agricultural Science
- Microbiology
- Biotechnology
Background:
- Feedlot waste presents a significant disposal challenge.
- Nutrient-rich feedlot waste can be a substrate for microbial fermentation.
- Trichoderma viride is known for its ability to produce enzymes like cellulase.
Purpose of the Study:
- To evaluate the potential of batch fermentation of feedlot waste using Trichoderma viride.
- To assess the utilization of carbohydrates and nutrient content during fermentation.
- To characterize the cellulase production by Trichoderma viride in this substrate.
Main Methods:
- Feedlot waste was subjected to batch fermentation with Trichoderma viride.
- Carbohydrate utilization and nitrogen content were monitored.
- Cellulase enzyme elaboration was quantified and compared to commercial standards.
- Organic matter reduction was measured.
Main Results:
- Trichoderma viride utilized two-thirds of the available carbohydrates in the feedlot waste.
- Cellulase was produced in quantities comparable to commercial preparations.
- The fermented waste was essentially odor-free.
- All original nitrogen was retained, with a 24% reduction in organic matter.
Conclusions:
- Batch fermentation with Trichoderma viride is an effective method for valorizing feedlot waste.
- This process offers a sustainable approach to waste management and enzyme production.
- The fermented product shows potential as a nutrient-rich, reduced-volume material.
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