Related Experiment Video
Updated: Aug 14, 2026

A Modified QuEChERS-HPLC Method for Detection of Polycyclic Aromatic Hydrocarbons in Zebrafish Embryos Exposed to Fine Particulate Matter
Published on: June 13, 2025
[Determination of the dissociation constants of basic compounds by reversed-phase high performance liquid
Qinghe Zhang1, Weibing Zhang, Tong Li
1National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116023, China. qinghe_zhang@elitehplc.com
Abstract:
The high performance liquid chromatographic (HPLC) behavior of aromatic amines and pyridine derivatives was studied on a new reversed-phase stationary phase, C12-alkyl-modifled zirconia. The effects of mobile phase variables such as methanol content and pH were investigated. The results showed that the stationary phase was stable in the mobile phase with a pH range of 2 - 12. It was found that on the new stationary phase the bases demonstrated the "traditional" behavior of retention due to their hydrophobicity. The retention factors of these basic compounds on the stationary phase increased rapidly with pH from pH 2 -7, and then increased slowly until reaching a constant value. The dissociation constants (pKa) of 13 basic compounds were determined based on the relationship between their retention factors and the pH of mobile phase. Compared with references, the errors of the pKa values obtained by this HPLC method were within 0.35 pH units.
More Related Videos
09:46Adsorption Device Based on a Langatate Crystal Microbalance for High Temperature High Pressure Gas Adsorption in Zeolite H-ZSM-5
Published on: August 25, 2016
10:33Development of Inhibitors of Protein-protein Interactions through REPLACE: Application to the Design and Development Non-ATP Competitive CDK Inhibitors
Published on: October 26, 2015
Related Concept Videos
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
Ion-Exchange Chromatography
Silica Gel Column Chromatography: Overview
Polar components tend to bind strongly to the silica gel, causing them to move slowly through the column. In contrast, nonpolar compounds...
High-Performance Liquid Chromatography: Instrumentation
Analyte Adsorption and Distribution
High-Performance Liquid Chromatography: Elution Process