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Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
Published on: October 17, 2013
Effect of radiocontrasts on selected membrane transport systems
Rubens Marona de Oliveira1, Bartira Ercília Pinheiro da Costa, Fernando Custódio Fervenza
1Programa de Pós-Graduação em Medicina e Ciências da Saúde (Nefrologia), Faculdade de Medicina, Hospital São Lucas e Instituto de Pesquisas Biológicas da Pontifícia, Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil. marona@pucrs.br
Background:
This study was aimed at examining the effects of radiocontrast agents on selected membrane transport functions.
Methods:
The effect of diatrizoate sodium (DS), diatrizoate meglumine (DM), and diatrizoate compound (DC) on Na+/K+ pump activity and number, L-arginine, and choline transports were evaluated in erythrocytes of normal individuals and patients undergoing cardiac catheterization.
Results:
Norrmal individuals' erythrocytes potassium influxes were 1.50+/-0.35, 1.32+/-0.37, 1.28+/-0.30, and 1.01+/-0.25 mmol/L cell/h in control, DS, DM, and DC groups, respectively (P=0.004; DC vs. control). Patients exposure to Hypaque M-76 significantly diminished Na+/K+ pump activity (1.40+/-0.36 before, vs. 1.27+/-0.40 mmol/L cell/h after; P=0.039). The number of Na+/K+ pumps was reduced (156+/-36 vs. 143+/-34 pumps/erythrocyte; P=0.015) in presence of DS. L-arginine and choline transports changed only at high radiocontrast concentrations.
Conclusion:
Selective changes in erythrocytes membrane transport function take place on exposure to radiocontrasts.
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