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Related Experiment Videos

Evidence for clonal evolution among highly polymorphic genes in methicillin-resistant Staphylococcus aureus.

G Kuhn1, P Francioli, D S Blanc

  • 1Hospital Preventive Medicine, University Hospital of Lausanne (CHUV), Avenue du Bugnon 48, 1011 Lausanne, Switzerland. Gerrit.Kuhn@chuv.ch.

Journal of Bacteriology
|December 15, 2005
PubMed
Summary

Staphylococcus aureus evolution is mainly clonal, even with highly variable adhesion genes. Recombination within clonal complexes may also shape its development.

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Area of Science:

  • Microbiology
  • Evolutionary Biology
  • Genetics

Background:

  • Staphylococcus aureus evolution is traditionally viewed as clonal, based on limited genetic markers.
  • The role of more variable genes in its evolutionary history remains less understood.

Purpose of the Study:

  • To investigate the evolutionary patterns of Staphylococcus aureus using both housekeeping and highly polymorphic adhesion genes.
  • To determine if epidemic and sporadic strains exhibit different evolutionary trajectories.

Main Methods:

  • Comparative analysis of partial gene sequences from 60 Staphylococcus aureus isolates, including methicillin-resistant strains.
  • Sequencing of seven housekeeping genes, six core adhesion genes, and four accessory adhesion genes.
  • Phylogenetic analysis and assessment of nucleotide diversity and selection pressures.

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Main Results:

  • Adhesion genes exhibited significantly higher nucleotide diversity (2- to 10-fold) compared to housekeeping genes.
  • All analyzed genes showed evidence of purifying selection, with accessory adhesion genes having a slightly reduced level.
  • Phylogenetic trees indicated overall clonality, but with evidence of homologous exchange, particularly within clonal complexes.
  • No significant differences in molecular evolution were observed between epidemic and sporadic strains for the highly variable genes.

Conclusions:

  • Staphylococcus aureus exhibits an overall clonal population structure, even when considering highly variable adhesion genes.
  • Recombination events within closely related isolates (clonal complexes) likely contribute to the species' evolution.
  • The study highlights the complex interplay between clonality and recombination in shaping Staphylococcus aureus diversity.