Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hydrogen bonding increases packing density in the protein interior.

David Schell1, Jerry Tsai, J Martin Scholtz

  • 1Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas 77843-2128, USA.

Proteins
|December 15, 2005
PubMed
Summary

Hydrogen bonds increase protein packing density by reducing the volume of buried polar groups. This finding helps reconcile theoretical models with experimental observations of protein stability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photoaging: Update on Pathogenesis, Prevention, and Treatment.

Annals of dermatology·2026
Same author

A 3D multi-compartment assembloid to study combined immune cell infiltration and cytotoxicity.

Cell reports methods·2026
Same author

Combined assembloid modeling and 3D whole-organ mapping captures the microanatomy and function of the human fallopian tube.

Science advances·2024
Same author

Health-related Quality of Life of Patients with Rosacea: A Systematic Review and Meta-analysis of Real-world Data.

Acta dermato-venereologica·2024
Same author

A 3D in vitro assay to study combined immune cell infiltration and cytotoxicity.

bioRxiv : the preprint server for biology·2024
Same author

Reinforcing Photoprotection for Skin of Color: A Narrative Review.

Dermatology and therapy·2023

Area of Science:

  • Biochemistry
  • Structural Biology
  • Computational Biology

Background:

  • The role of hydrogen bonds and buried polar groups in protein stability is debated.
  • Theoretical models often predict unfavorable stability contributions from buried polar groups, contrasting with experimental findings.

Purpose of the Study:

  • To investigate how hydrogen bonds influence packing density within protein interiors.
  • To reconcile discrepancies between theoretical predictions and experimental data on protein stability.

Main Methods:

  • Analysis of 687 crystal structures.
  • Quantification of the volume occupied by buried polar groups based on their hydrogen bonding extent.

Main Results:

  • Hydrogen-bonded peptide groups and polar side chains occupy less volume than non-hydrogen-bonded ones.

Related Experiment Videos

  • Peptide groups with complete hydrogen bonding were found to occupy 5.2 ų less volume on average.

    • Conclusions:

    • Hydrogen bonds contribute to protein stability, potentially by increasing packing density.
    • The findings suggest that improved theoretical models should account for hydrogen bond-mediated packing effects.