Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Revealing posttranscriptional regulatory elements through network-level conservation.

Chang S Chan1, Olivier Elemento, Saeed Tavazoie

  • 1Department of Molecular Biology and The Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA.

Plos Computational Biology
|December 16, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Respiration defects limit serine synthesis required for lung cancer growth and survival.

Nature communications·2025
Same author

Immune Checkpoint Blockade Delays Cancer Development and Extends Survival in DNA Polymerase Mutator Syndromes.

Cancer research·2025
Same author

The power and perils of large language models in haematology.

British journal of haematology·2024
Same author

Detection of rare and novel gene fusions in patients with diffuse glioma: An institutional retrospective study.

Journal of neuropathology and experimental neurology·2024
Same author

Deficiency of metabolic regulator PKM2 activates the pentose phosphate pathway and generates TCF1<sup>+</sup> progenitor CD8<sup>+</sup> T cells to improve immunotherapy.

Nature immunology·2024
Same author

Automatic ploidy prediction and quality assessment of human blastocysts using time-lapse imaging.

Nature communications·2024

Conserved mRNA motifs in fruit flies and worms reveal microRNA (miRNA) targets and regulatory networks. This study identifies novel miRNA targets and insights into gene regulation across species.

Area of Science:

  • Comparative genomics
  • Post-transcriptional regulation
  • Molecular evolution

Background:

  • MicroRNAs (miRNAs) are key regulators of gene expression.
  • Identifying miRNA targets is crucial for understanding cellular function.
  • Conserved non-coding elements can reveal functional regulatory sites.

Purpose of the Study:

  • To identify conserved mRNA motifs in 3' untranslated regions (UTRs) of fly and worm genomes.
  • To discover novel microRNA (miRNA) target sites and RNA-binding protein sites.
  • To investigate the evolution and combinatorial nature of post-transcriptional regulatory networks.

Main Methods:

  • Network-level genomic conservation analysis between Drosophila and Caenorhabditis species.
  • Identification of conserved mRNA motifs complementary to known miRNAs.

Related Experiment Videos

  • Genome-wide search for conserved stem-loops and novel candidate miRNAs.

    • Main Results:

    • Detected highly conserved mRNA motifs in 3' UTRs, many corresponding to miRNA target sites.
    • Identified known and novel functional sites for RNA-binding proteins.
    • Found that genes with similar functions often share conserved regulatory elements.
    • Observed simultaneous conservation of target sites for multiple miRNAs, indicating combinatorial regulation.
    • Discovered numerous new candidate miRNAs targeting novel sites.

    Conclusions:

    • Conserved elements in 3' UTRs are rich in miRNA and RNA-binding protein regulatory sites.
    • Combinatorial miRNA regulation is a common feature in these species.
    • Post-transcriptional networks show extensive rewiring across phyla, despite conserved regulatory elements.