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Updated: Jul 28, 2026
Cheek Injection Model for Simultaneous Measurement of Pain and Itch-related Behaviors
Published on: September 27, 2019
Scratching behavior in mice induced by the proteinase-activated receptor-2 agonist, SLIGRL-NH2
Steven G Shimada1, Kelly A Shimada, J G Collins
1Department of Anesthesiology, Yale University School of Medicine, P.O. Box 208051, 333 Cedar Street, New Haven, CT 06520-8051, USA. steven.shimada@yale.edu
Abstract:
We examined whether the proteinase-activated receptor-2 (PAR2) agonist, H-Ser-Leu-Ile-Gly-Arg-Leu-NH2 (SLIGRL-NH2), could induce scratching behavior in mice. Intradermal injections of SLIGRL-NH2 (10-50 microg) evoked dose dependent scratching. This behavior peaked near 5 min and returned to preinjection levels within 30 min. Pretreatment of animals with a histamine H1 receptor antagonist, pyrilamine, blocked histamine induced scratching, but it had little effect on SLIGRL scratching. Our study suggests that PAR2 mediates histamine independent itch.
