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Related Experiment Videos

Non-conventional protein secretion in yeast.

César Nombela1, Concha Gil, W LaJean Chaffin

  • 1Departamento de Microbiología, Facultad de Farmacia, Universidad Complutense, 28040-Madrid, Spain. cnombela@farm.ucm.es

Trends in Microbiology
|December 17, 2005
PubMed
Summary

Many yeast proteins reach the cell surface without a signal peptide, suggesting alternative secretion pathways. These "moonlighting" proteins may play roles in virulence and cell-wall dynamics.

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Area of Science:

  • Microbiology
  • Cell Biology
  • Biochemistry

Background:

  • Proteins are transported to the cell surface in Saccharomyces cerevisiae and Candida albicans for cell-wall integration or export.
  • Classical secretion relies on an N-terminal signal peptide via the endoplasmic reticulum-Golgi pathway.
  • Some yeast surface proteins lack signal peptides but still reach the cell exterior.

Purpose of the Study:

  • To investigate the alternative secretion mechanisms for yeast cell surface proteins lacking canonical signal peptides.
  • To explore the potential "moonlighting" functions of these signal-less proteins.
  • To understand the implications of alternative secretion in yeast virulence and cell-wall dynamics.

Main Methods:

  • Analysis of yeast protein localization.

Related Experiment Videos

  • Identification of proteins lacking N-terminal signal peptides.
  • Investigation of non-classical secretion pathways.

    • Main Results:

    • Several yeast proteins, including glycolytic enzymes and chaperones, are found on the cell surface without a signal peptide.
    • These signal-less proteins are identified as potentially "moonlighting" (multifunctional).
    • Evidence suggests non-classical secretion routes transport these proteins beyond the plasma membrane.

    Conclusions:

    • Alternative secretion pathways exist in yeasts for proteins lacking signal peptides.
    • These signal-less proteins may have dual functions, contributing to cellular processes and potentially virulence.
    • Further research into alternative secretion is crucial for understanding yeast biology and pathogenesis.