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Published on: November 23, 2015
Remote NADH imaging through an ordered array of electrochemiluminescent nanoapertures
Arnaud Chovin1, Patrick Garrigue, Neso Sojic
1Laboratoire d'Analyse Chimique par Reconnaissance Moléculaire, Université Bordeaux I, ENSCPB, 16 avenue Pey-Berland, 33607 Pessac, France.
Bioelectrochemistry (Amsterdam, Netherlands)
|December 17, 2005
Summary
We developed a nanoaperture array for sensitive electrochemiluminescent (ECL) detection of NADH. This technology enables remote, spatially resolved NADH concentration measurements with high resolution.
Area of Science:
- Electrochemistry
- Optical sensing
- Nanotechnology
Background:
- Nicotinamide adenine dinucleotide (NADH) is a crucial biomarker for various biological processes.
- Existing NADH detection methods often require complex sample handling and lack spatial resolution.
- Electrochemical and optical detection methods offer potential for sensitive and specific NADH quantification.
Purpose of the Study:
- To develop a novel nanoaperture array for sensitive and spatially resolved electrochemiluminescent (ECL) detection of NADH.
- To integrate electrochemical generation of light, optical collection, and remote imaging functionalities into a single device.
- To investigate the ECL mechanism of NADH oxidation mediated by Ru(bpy)3(2+) on a gold nanoring electrode array.
Main Methods:
- Fabrication of a high-density array of nanoapertures on a coherent optical fiber bundle, with each aperture surrounded by a gold nanoring electrode.
- Characterization of the array's electrochemical properties using cyclic voltammetry.
- Measurement of ECL intensity generated from NADH oxidation in the presence of Ru(bpy)3(2+) as a function of NADH concentration and applied potential.
- Remote imaging of the ECL signal using a CCD camera on the proximal face of the optical fiber bundle.
Main Results:
- The nanoaperture array exhibited excellent electrochemical performance.
- A linear relationship was observed between ECL intensity and NADH concentration.
- The system demonstrated remote, spatially resolved detection of NADH with micrometer resolution.
- The influence of applied potential on ECL intensity was investigated, providing insights into the NADH ECL mechanism on gold surfaces.
Conclusions:
- The developed nanoaperture array successfully integrates ECL light generation, collection, transmission, and remote imaging.
- This technology offers a promising platform for sensitive, spatially resolved, and remote detection of NADH.
- The findings pave the way for advanced biosensing applications requiring high-resolution electrochemical imaging.
