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Myricetin stimulates the absorption of the pro-carcinogen PhIP
Maaike E Schutte1, Johannes J M van de Sandt, Gerrit M Alink
1Division of Toxicology, Wageningen University, P.O. Box 8000, 6700 EA Wageningen, The Netherlands. Maaike.schutte@wur.nl
Abstract:
The effect of the flavonoid myricetin on the transport of the pro-carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) through differentiated Caco-2 monolayers, a model for the intestinal epithelium, is described. Myricetin causes an increase of the transport of PhIP from the apical to the basolateral compartment. This effect was observed at physiologically relevant concentrations of PhIP and myricetin. Cyclosporin A (MRP2 inhibitor) but not PSC833 (P-gp inhibitor) showed a similar effect on PhIP transport. The results indicate that myricetin induces an increased basolateral uptake of the pro-carcinogen PhIP, in part through inhibition of the MRP2 mediated excretion of PhIP from the intestinal cells back to the lumen.
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