Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The laboratory in a droplet.

Helen M O'Hare1, Kai Johnsson

  • 1Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

Chemistry & Biology
|December 17, 2005
PubMed
Summary

Researchers developed a new method using fluorescence-activated cell sorting of double emulsions for screening enzyme activity. This technique significantly enhances enzyme screening throughput, making it two orders of magnitude faster.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Localizable Fluorescent Metal Ion Indicators With Tunable Colors.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Real-Life Application of a Point-of-Care Biosensor for Phenylalanine in Patients With Phenylketonuria.

Journal of inherited metabolic disease·2026
Same author

A high-affinity split-HaloTag for live-cell protein labeling.

Nature communications·2026
Same author

Fast, Bright, and Reversible Fluorescent Labeling of Rhodamine-Binding Proteins.

Journal of the American Chemical Society·2025
Same author

A Chemically Switchable Synthetic Condensate Platform for Reversible Protein Sequestration and Release.

ACS chemical biology·2025
Same author

SiR-XActin: A Fluorescent Probe for Imaging Actin Dynamics in Live Cells.

Angewandte Chemie (International ed. in English)·2025

Area of Science:

  • Biochemistry
  • Chemical Biology
  • Biotechnology

Background:

  • Enzyme activity screening is crucial for various biological and chemical applications.
  • Current enzyme screening methods can be limited by throughput and efficiency.

Purpose of the Study:

  • To present a novel, generally applicable method for high-throughput enzyme activity screening.
  • To demonstrate the utility of fluorescence-activated cell sorting of double emulsions for this purpose.

Main Methods:

  • Utilized fluorescence-activated cell sorting (FACS) combined with double emulsion technology.
  • Developed a screening platform capable of analyzing individual droplets within emulsions.

Main Results:

  • Achieved a two-orders-of-magnitude increase in throughput compared to typical enzyme screens.
  • Demonstrated the general applicability of the developed methodology across different enzyme activities.

Conclusions:

  • Fluorescence-activated cell sorting of double emulsions offers a powerful and efficient approach for enzyme activity screening.
  • This novel methodology significantly advances the capabilities for enzyme discovery and characterization.

Related Experiment Videos