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Updated: Aug 14, 2026
A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
Published on: August 8, 2013
Determination of theophylline concentration in serum by chemiluminescent immunoassay
Mei-xia Zhou1, Cha-ying Guan, Guang Chen
1Department of Laboratory, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou 310016, China. zmx00018@126.com
Objective:
This study aimed to establish chemiluminescent immunoassay (CLIA) for quantitative determination of theophylline levels in human serum.
Methods:
To measure the concentration of theophylline (n=122) and evaluate the assay.
Results:
The linear range of the CLIA method was 0.51-40 mg/L (Y=1.02X+0.44, r=0.995). The intra and inter CV (coefficient variance) of CLIA were 3.20% and 3.57%, respectively. The average recovery rate was 102.3%. This method was free from interference by brilirubin (<200 micromol/L), hemoglobin (<10 g/L), and triglycerides (<15 mmol/L).
Conclusion:
This method is simple, convenient and precise for clinical pharmacokinetics study of theophylline.
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