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Conjunctival impression cytology in dogs.
Aline A Bolzan1, Adriana T J Brunelli, Márcio B Castro
1Faculdade de Ciências Agrárias e Veterinárias-UNESP, Jaboticabal, São Paulo, Brazil.
Veterinary Ophthalmology
|December 20, 2005
Summary
Conjunctival impression cytology is a feasible technique for sampling dog eyes. This study standardized the protocol and identified normal cytologic patterns in healthy canine conjunctivas.
Area of Science:
- Veterinary Ophthalmology
- Cytopathology
Background:
- Conjunctival impression cytology is a valuable diagnostic tool.
- Standardization is crucial for reliable results in canine ophthalmology.
Purpose of the Study:
- Evaluate the feasibility of conjunctival impression cytology in dogs.
- Standardize a protocol for canine conjunctival sampling.
- Recognize normal cytologic patterns of the canine conjunctiva.
Main Methods:
- Collected conjunctival samples from 20 healthy dogs using topical anesthesia.
- Applied Millipore filter strips to the bulbar conjunctiva.
- Fixed, stained (PAS and Hematoxylin), and microscopically examined samples.
Main Results:
- Identified superficial, intermediate, and basal epithelial cells in sheets.
- Observed keratinized cells, goblet cells, leukocytes, debris, and mucus.
- Demonstrated the characteristic cytologic features of healthy canine conjunctiva.
Conclusions:
- Confirmed the feasibility of impression cytology for canine bulbar conjunctiva.
- Established a standardized protocol for this technique.
- Enabled recognition of normal cytologic patterns in healthy dogs.