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Related Experiment Videos

Release of cyclic AMP by toad urinary bladder.

S Urakabe, J S Handler, J Orloff

    The American Journal of Physiology
    |March 1, 1975
    PubMed
    Summary
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    This study shows cyclic adenosine monophosphate (cAMP) is released from toad bladder epithelial cells. Vasopressin increases cAMP, while theophylline may reduce its release, and NaF blocks vasopressin's water permeability effect.

    Area of Science:

    • Physiology
    • Cell Biology
    • Biochemistry

    Background:

    • Cyclic adenosine monophosphate (cAMP) is a crucial second messenger in cellular signaling.
    • Understanding cAMP dynamics in epithelial tissues is vital for comprehending physiological responses.

    Purpose of the Study:

    • To investigate the release and origin of cyclic AMP (cAMP) in the toad urinary bladder.
    • To examine the effects of vasopressin and theophylline on cAMP levels and release.
    • To elucidate the mechanism of action of NaF on vasopressin-induced water permeability.

    Main Methods:

    • In vitro incubation of toad urinary bladder in Ringer solution.
    • Measurement of cyclic AMP (cAMP) accumulation in serosal and mucosal bathing solutions.
    • Assessment of intracellular cAMP levels in epithelial cells and stroma.

    Related Experiment Videos

  • Pharmacological manipulation using vasopressin, theophylline, and sodium fluoride (NaF).

    • Main Results:

    • Cyclic AMP (cAMP) accumulates in the Ringer solution, with significantly higher release into the serosal than mucosal solution.
    • The majority of released cAMP originates from epithelial cells.
    • Vasopressin elevates intracellular cAMP and increases its release into the bathing solution.
    • Theophylline increases intracellular cAMP but not medium cAMP, suggesting reduced membrane permeability.
    • Sodium fluoride (NaF) increases intracellular and medium cAMP but inhibits vasopressin's effect on water permeability.

    Conclusions:

    • Toad urinary bladder epithelial cells release cyclic AMP (cAMP) primarily into the serosal compartment.
    • Vasopressin stimulates cAMP production and release, while theophylline may impede its efflux.
    • NaF interferes with vasopressin signaling downstream of cAMP production, impacting water permeability.