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Epinephrine quantification in pharmaceutical formulations utilizing plant tissue biosensors
Fabiana S Felix1, Miyuki Yamashita, Lúcio Angnes
1Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508-900 São Paulo, SP, Brazil.
Biosensors & Bioelectronics
|December 20, 2005
Summary
A novel plant tissue biosensor using Livistona chinensis enzymes offers a simple and effective method for quantifying epinephrine in pharmaceuticals. This electrochemical approach provides accurate results, unaffected by common pharmaceutical additives.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Electrochemistry
Background:
- Epinephrine quantification is crucial for pharmaceutical analysis.
- Existing methods may be affected by sample matrices.
- Development of sensitive and selective biosensors is needed.
Purpose of the Study:
- To develop and validate a plant tissue biosensor for epinephrine determination.
- To utilize polyphenol oxidase enzymes from Livistona chinensis for epinephrine detection.
- To assess the biosensor's performance in pharmaceutical samples.
Main Methods:
- Flow injection analysis coupled with a plant tissue biosensor.
- Electrochemical detection of epinephrine oxidation product at -0.10 V vs. Ag/AgCl.
- Enzymatic catalysis by polyphenol oxidase from Livistona chinensis.
Main Results:
- Linear response for epinephrine from 5.0 x 10(-5) to 3.5 x 10(-4) mol L(-1).
- Limit of detection of 1.5 x 10(-5) mol L(-1) with a correlation coefficient of 0.998.
- Repeatability of 3.1% (R.S.D.) and good agreement with UV spectrophotometry and plant tissue reactor methods.
Conclusions:
- The proposed plant tissue biosensor is a simple, sensitive, and reliable tool for epinephrine quantification.
- The method is robust and not affected by benzoic acid, unlike the official spectrophotometric procedure.
- This biosensor offers an attractive alternative for routine pharmaceutical analysis of epinephrine.