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Published on: July 21, 2014
Transcriptional regulators à la carte: engineering new effector specificities in bacterial regulatory proteins
Teca Calcagno Galvão1, Víctor de Lorenzo
1Department of Microbial Biotechnology, Centro Nacional de Biotecnología-CSIC, Madrid 28049, Spain. tgalvao@cnb.uam.es
Abstract:
For many regulators of bacterial biodegradation pathways, small molecule/effector binding is the signal for triggering transcriptional activation. Thus, regulation results from a cross-talk between chemicals sensed by transcriptional factors and operon expression status. These features can be utilised in the construction of biosensors for a wide range of target compounds as, in principle, any regulatory protein whose activity is modulated by binding to a small molecule can have its effector/inducer profile artificially altered. The cognate specificities of a number of regulatory proteins have been modified as an astute approach to developing, among others, bacterial biosensors for environmentally relevant compounds.
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