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Updated: Aug 14, 2026

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Published on: December 8, 2023
A derivative of Mycobacterium smegmatis mc(2)155 that lacks the duplicated chromosomal region
Digby F Warner1, Gilles Etienne, Xiao-Ming Wang
1MRC/NHLS/WITS Molecular Mycobacteriology Research Unit, DST-NRF, School of Pathology of the University of the Witwatersrand, Johannesburg, South Africa. digby.warner@nhls.ac.za <digby.warner@nhls.ac.za>
Abstract:
The genome of Mycobacterium smegmatis mc(2)155 contains a 56kb duplicated region. We isolated a mutant of mc(2)155 lacking this duplication (DeltaDRKIN). This mutation did not affect the growth rate, surface properties or transformation efficiency of the organism, confirming the potential utility of DeltaDRKIN for the study of genes contained within the duplicated region.
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