Related Experiment Video
Updated: Aug 14, 2026
Chromosome Screening of Human Preimplantation Embryos by Using Spent Culture Medium: Sample Collection and Chromosomal Ploidy Analysis
Published on: September 7, 2021
Intracytoplasmic sperm injection: a novel selection method for sperm with normal frequency of chromosomal
Attila Jakab1, Denny Sakkas, Elena Delpiano
1Sperm Physiology Laboratory, Yale University School of Medicine, New Haven, Connecticut 06520, USA.
Objective:
To test a newly invented intracytoplasmic sperm injection (ICSI) sperm selection method based on sperm hyaluronic acid (HA) binding.
Design:
Comparison of chromosomal disomy and diploidy frequencies in sperm arising from semen and in HA-bound sperm.
Setting:
Academic andrology laboratory.
Patient(S):
Men presenting for semen analysis.
Intervention(S):
Washed sperm fractions of 32 semen samples were applied to Petri dishes or glass slides coated with immobilized HA. The unbound sperm were rinsed gently, and the HA-bound sperm were removed with an ICSI pipette. The control sperm population was the unselected sperm. Both HA-selected and unselected sperm were treated with fluorescence in situ hybridization with centromeric probes for the X, Y, and 17 chromosomes.
Main Outcome Measure(S):
Chromosomal disomy and diploidy frequencies.
Result(S):
In the HA-bound sperm (495-2,079 per man, 41,670 in all) compared with unselected sperm (4,770 per man, 162,210 in all), the chromosomal disomy frequencies were reduced to 0.16% from 0.52%, diploidy to 0.09% from 0.51%, and sex chromosome disomy to 0.05% from 0.27% (a 5.4-fold reduction vs. 4-fold respective increase in ICSI offspring).
Conclusion(S):
The HA sperm selection method for ICSI, which is based on a relationship between sperm receptors for zona pellucida and HA, will likely reduce the potential genetic complications and adverse public health effects of ICSI.
