Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Organization of interphase chromatin.

Rachel A Horowitz-Scherer1, Christopher L Woodcock

  • 1Biology Department and Molecular and Cellular Biology Program, University of Massachusetts at Amherst, 01003, USA.

Chromosoma
|December 20, 2005
PubMed
Summary

This review explores chromatin organization, integrating molecular and nuclear data. Emerging technologies aim to bridge the resolution gap between microscopy and biophysical methods for a comprehensive understanding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hierarchical looping of zigzag nucleosome chains in metaphase chromosomes.

Proceedings of the National Academy of Sciences of the United States of America·2016
Same author

Sumoylated human histone H4 prevents chromatin compaction by inhibiting long-range internucleosomal interactions.

The Journal of biological chemistry·2014
Same author

Comparative structure of vertebrate sperm chromatin.

Journal of structural biology·2014
Same author

Human heterochromatin protein 1α promotes nucleosome associations that drive chromatin condensation.

The Journal of biological chemistry·2014
Same author

Nucleosome-free region dominates histone acetylation in targeting SWR1 to promoters for H2A.Z replacement.

Cell·2013
Same author

Chromatin organization - the 30 nm fiber.

Experimental cell research·2012

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biophysics

Background:

  • Interphase chromatin organization spans molecular to whole-nucleus scales.
  • Diverse experimental approaches are required to study chromatin structure.
  • Integrating data across different scales presents a significant challenge.

Purpose of the Study:

  • To review experimental approaches for studying chromatin organization.
  • To examine the results and interfaces between different methodologies.
  • To discuss strategies and emerging technologies for data integration.

Main Methods:

  • Light microscopy for whole nuclei imaging.
  • Biophysical characterization, atomic force microscopy, and electron microscopy for in vitro studies.
  • Comparative analysis of diverse experimental techniques.

Main Results:

  • Current methods provide insights at molecular and nuclear levels but lack seamless integration.
  • A 'resolution gap' exists between light microscopy and high-resolution in vitro techniques.
  • Strategies for integrating multi-scale data are being developed.

Conclusions:

  • Integrating multi-scale chromatin data is crucial for understanding nuclear organization.
  • Emerging technologies show promise in bridging the resolution gap.
  • Further research is needed to unify diverse experimental findings.

Related Experiment Videos