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Updated: Aug 14, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Structural and dynamical analyses of the kinase network derived from the transpath database
Bernd Binder1, Reinhart Heinrich
1Theoretical Biophysics, Institute of Biology, Humboldt University Berlin, Berlin, Germany. bernd.binder@rz.hu-berlin.de
Abstract:
We analyze the structural design and the dynamical properties of a protein kinase network derived from the Transpath database. We consider structural properties, such as feedback cycles, pathway lengths, fraction of shortest pathways and crosstalk. Dynamic characteristics of the network are analyzed by using nonlinear differential equations with a special focus on kinase amplitudes and signal propagation times. Comparison with random networks shows that the cellular kinase network exhibits special features which might be a result of natural selection. In particular, the Transpath network contains no cycles, and input kinases and output kinases are generally connected by shortest signalling routes. Moreover, it displays a characteristic spectrum of cross-talk between different pathways.
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