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Flow-injection determination of ascorbic acid and cysteine simultaneously with spectrofluorometric detection.
Behzad Rezaei1, Ali Asghar Ensafi, Siavash Nouroozi
1College of Chemistry, Isfahan University of Technology, Iran.
Summary
A new spectrofluorometric method enables simultaneous determination of ascorbic acid and cysteine using a flow-injection system. This sensitive technique accurately quantifies these compounds in real samples.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Ascorbic acid (vitamin C) and cysteine are vital biomolecules.
- Accurate simultaneous quantification is crucial for various applications.
- Existing methods may lack sensitivity or specificity.
Purpose of the Study:
- Develop a simple, sensitive, and simultaneous spectrofluorometric method.
- Quantify ascorbic acid and cysteine in real samples.
- Utilize a flow-injection system for efficiency.
Main Methods:
- Flow-injection analysis coupled with spectrofluorometry.
- Reduction of Thallium(III) by ascorbic acid and cysteine to form a fluorescent reagent.
- Differential measurement utilizing two separate sample streams.
- Spectrofluorometric detection at specific excitation and emission wavelengths (λex = 227 nm, λem = 419 nm).
Main Results:
- Simultaneous determination of ascorbic acid and cysteine achieved.
- Linear determination ranges: 1 x 10⁻⁶ to 5.0 x 10⁻⁵ M.
- Detection limits (S/N=3): 8 x 10⁻⁷ M for ascorbic acid and 7 x 10⁻⁷ M for cysteine.
- Analysis rate of 16 samples per hour.
- Successful application to real samples with minimal interference.
Conclusions:
- The developed flow-injection spectrofluorometric method is highly sensitive and selective.
- It provides a reliable approach for simultaneous determination of ascorbic acid and cysteine.
- The method's simplicity and efficiency make it suitable for routine analysis.