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Updated: Aug 12, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
Health of infants in an Imperial Roman skeletal sample: perspective from dental microstructure
Charles FitzGerald1, Shelley Saunders, Luca Bondioli
1Department of Anthropology, McMaster University, Hamilton, Ontario L8S 4L9, Canada. cfitzg@mcmaster.ca
Insights
This study analyzed microscopic enamel defects in Roman children
Area of Science:
- Paleopathology
- Bioarchaeology
- Human Osteology
Background:
- Assessing general health in past populations is crucial for understanding historical living conditions.
- Microscopic enamel defects, such as Wilson bands, offer a precise method to evaluate early childhood health.
- The Imperial Roman necropolis of Isola Sacra provides a unique dataset for this analysis.
Observation:
- 274 deciduous teeth from 127 subadults (2nd-3rd century ACE) were analyzed for Wilson bands.
- Microstructural enamel markers were used to precisely date Wilson band formation.
- Maximum prevalence (MAP) and smoothed maximum prevalence (SMAP) distributions were calculated.
Findings:
- Two distinct periods of high Wilson band prevalence were identified in Roman infants.
- The first period of high prevalence occurred from 2 to 5 months of age.
- A second, more significant period of high prevalence occurred from 6 to 9 months of age.
Implications:
- These findings suggest critical periods of physiological stress during infancy in Roman childhood.
- The study provides a realistic health risk profile for Roman children.
- Results can inform our understanding of Roman childhood rearing practices and their impact on health.
Abstract:
This study examines general health in the first year of life of a population of 127 subadults from the Imperial Roman necropolis of Isola Sacra (2nd-3rd century ACE). Health status was determined by analyzing 274 deciduous teeth from these children for Wilson bands (also known as accentuated striae), microscopic defects caused by a disruption to normal enamel development arising from some generalized external stressor. While macroscopic enamel defects, or hypoplasias, have long been used as proxies of general population health, we believe that this is the first population-wide study of microscopic defects in deciduous teeth. We used microstructural markers of enamel to attach very precise chronologies to Wilson band formation that allowed us to calculate maximum prevalence (MAP) and smoothed maximum prevalence (SMAP) distributions to portray what we believe to be a realistic risk profile for a past population of children. There appear to be two periods of high prevalence, the first beginning around age 2 months and continuing through month 5, and the second higher period beginning around month 6 and continuing through month 9. These results are discussed in light of historical records of Roman childhood rearing practices.
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