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Related Experiment Videos

Light-induced exocytosis in cell development and differentiation.

Andreas W Henkel1, Irene Upmann, Christina R Bartl

  • 1Department of Psychiatry, University of Erlangen-Nuremberg, Schwabachanlage 6, 91054 Erlangen, Germany. andreas.henkel@psych.imed.uni-erlangen.de

Journal of Cellular Biochemistry
|December 21, 2005
PubMed
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Light can trigger calcium-dependent exocytosis in cells, identified as a mechanism for plasma membrane building during development, not neurotransmitter release.

Area of Science:

  • Cell Biology
  • Neuroscience
  • Developmental Biology

Background:

  • Exocytosis is a fundamental cellular process involving vesicle fusion with the plasma membrane.
  • Calcium ions are known regulators of exocytosis, particularly in neuronal signaling.
  • The role of light in regulating exocytosis in non-photoreceptor cells is not well understood.

Purpose of the Study:

  • To investigate the phenomenon of light-induced exocytosis in PC12 and primary embryonic telencephalon cells.
  • To identify the molecular mechanisms and cellular roles of light-triggered exocytosis.
  • To differentiate between light-induced exocytosis for membrane construction versus neurotransmitter release.

Main Methods:

  • Utilizing fluorescently labeled secretory vesicles for imaging.

Related Experiment Videos

  • Employing Total Internal Reflection Fluorescence (TIRF) and epifluorescence microscopy.
  • Quantitative PCR (qPCR) expression analysis to identify the light receptor molecule.
  • Analyzing the frequency, time course, and spatial distribution of exocytotic events.

    • Main Results:

    • Visible light illumination successfully triggered calcium-dependent exocytosis in both cell types.
    • Opsin 3 was identified as the likely light receptor mediating this response.
    • Light-induced exocytosis occurred during a specific developmental window in primary cells, involving large vesicles.
    • Exocytosis events were observed at cell peripheries and interfaces, with variable durations.
    • The process showed a few seconds delay and lasted up to 2 minutes.

    Conclusions:

    • Light-induced exocytosis is a calcium-dependent process regulated by Opsin 3.
    • This mechanism appears to be primarily involved in plasma membrane construction during cell differentiation, development, and proliferation.
    • The findings suggest a novel role for light in cellular membrane dynamics, distinct from classical calcium-dependent neurotransmitter release.