Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The ubiquitin-specific protease USP10 modulates androgen receptor function.

Hortensia Faus1, Hellmuth-Alexander Meyer, Martina Huber

  • 1Corporate Research Oncology, Schering AG, D-13342 Berlin, Germany.

Molecular and Cellular Endocrinology
|December 22, 2005
PubMed
Summary

The ubiquitin-specific protease USP10 binds to the androgen receptor (AR) and enhances its activity. This discovery highlights the ubiquitin/proteasome system

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Temperature Dependence of Paramagnetic Species in the Human Brain Tissue: An X-Band EPR Study.

Magnetic resonance in medicine·2025
Same author

Identifying the Elusive Dimerization Product Interfering with Methylsulfonato-Group Labeling of Cysteines in Proteins.

ChemistryOpen·2025
Same author

Terminal spin labeling of xylotriose strongly affects interactions in the active site of xylanase BcX.

Journal of biomolecular NMR·2025
Same author

Inhibition of EphB4 Receptor Signaling by Ephrin-B2-Competitive and Non-Competitive DARPins Prevents Angiogenesis.

Biochemistry·2025
Same author

Darolutamide in Combination with Radium-223 Exhibits Synergistic Antitumor Efficacy in LNCaP Prostate Cancer Models.

International journal of molecular sciences·2025
Same author

Coupling Modelling and Experiments to Analyse Leaf Photosynthesis Under Far-Red Light.

Plant, cell & environment·2024

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • The ubiquitin/proteasome system regulates nuclear hormone receptor function.
  • Androgen receptor (AR) activity is crucial in various cellular processes.

Purpose of the Study:

  • To identify novel cofactors involved in androgen receptor (AR) transcriptional regulation.
  • To elucidate the role of the ubiquitin-specific protease USP10 in AR function.

Main Methods:

  • Purification of DNA-bound AR complexes from nuclear extracts.
  • GST pull-down assays to confirm protein interactions.
  • Fluorescence microscopy for subcellular localization.
  • Cell-based transactivation assays and siRNA knock-down experiments.

Related Experiment Videos

Main Results:

  • USP10 was identified as a component of DNA-bound AR complexes.
  • USP10 interacts with the AR and is localized in both nucleus and cytoplasm.
  • Overexpression of USP10 stimulates AR-mediated transactivation, while its knock-down impairs it.

Conclusions:

  • USP10 is a novel cofactor that binds to and stimulates the androgen receptor (AR).
  • This finding emphasizes the role of the ubiquitin/proteasome system in modulating AR transcriptional activity.