Related Experiment Video
Updated: Aug 14, 2026

Quantification of γH2AX Foci in Response to Ionising Radiation
Published on: April 6, 2010
Some aspects of quantitative analysis and correction of radiation damage
1Universität Konstanz, Fachbereich Biologie, M647, D-78457 Konstanz, Germany. kay.diederichs@uni-konstanz.de
Abstract:
Radiation damage is the major source of systematic error in macromolecular data collected at third-generation synchrotron beamlines. In this paper, a simple way of analysing data for radiation damage is proposed and shown to give results that are easy to interpret. Results of correction of radiation damage obtained with an exponential decay function as implemented in XSCALE (from the XDS package) are shown, and aspects of the mathematical treatment of radiation damage, as well as experimental requirements for the correction and utilization of radiation damage are discussed. Furthermore, a method for quantifying the coverage and evenness of sampling of rotation range is proposed.
Related Concept Videos
Biological Effects of Radiation
Quantitative Analysis
In quantitative analysis, two key measurements are made: the sample quantity and a property proportional to the amount of the analyte (the substance being analyzed). This forms the basis of the method...
Contaminants and Errors
Another key consideration is determining the appropriate number of samples required to...
Cancer Survival Analysis

