Comparative proteomic analysis of B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis
Related Experiment Video
Updated: Sep 18, 2025
Proteomic Profiling of Macrophages by 2D Electrophoresis
Published on: November 4, 2014
Su-Qing Zhao1, Yan-Fei Cai, Zhen-Yu Zhu
1Unite des Rickettsies, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 05, France. suqingz@hotmail.com
Objective:
To compare the protein difference between B. henselae Houston and B. henselae Marseille by two-dimensional gel electrophoresis.
Method:
Protein samples were prepared by vorterx, ultrasonic treatment, and centrifugation. Protein concentrations were determined by Bradford method. Protein difference was compared by the first IEF and the second SDS-polyacrylamide gel electrophoresis.
Results:
Protein concentrations in samples of Bartonella henselae Houston and Bartonella henselae Marseille were 2.117 microg/microL and 2.200 microg/microL respectively. Sample protein of 40 microg for IPG strips loading was perfect. The results of 2-DE in pH 4 to 7 IPG strips showed that the total protein spots of Bartonella henselae Houston and Bartonella henselae Marseille were 375 and 379 respectively, 95% of the spots were the same between the two strains of Bartonella henselae.
Conclusion:
The procedure of 2-DE may prove successful for the proteomic analysis of Bartonella henselae. Bartonella henselae Houston and Bartonella henselae Marseille are different genotypes.
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