Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mesenchymal stem cells: isolation, in vitro expansion and characterization.

N Beyer Nardi1, L da Silva Meirelles

  • 1Genetics Department, Universidade Federal do Rio Grande do Sul, Av Bento Gonçalves 9500, Porto Alegre RS, CEP 91540-000, Brazil. nardi@ufrgs.br

Handbook of Experimental Pharmacology
|December 24, 2005
PubMed
Summary

Mesenchymal stem cells (MSCs) are versatile adult stem cells with therapeutic potential but poorly understood properties. Further research is needed to clarify MSC identity and characteristics for optimal clinical application.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Stability and surgical complications of tooth-borne and bone-borne appliances in surgical assisted rapid maxillary expansion: a systematic review.

The British journal of oral & maxillofacial surgery·2021
Same author

Hierarchy of surgical stability in orthognathic surgery: overview of systematic reviews.

International journal of oral and maxillofacial surgery·2019
Same author

Stability and surgical complications in segmental Le Fort I osteotomy: a systematic review.

International journal of oral and maxillofacial surgery·2017
See all related articles

Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Mesenchymal stem cells (MSCs) are adult stem cells known for their ease of isolation, culture, and manipulation.
  • These cells exhibit significant plasticity and hold promise for diverse therapeutic applications, yet their fundamental properties remain largely undefined.
  • MSCs are found in various tissues, including bone marrow, and are typically identified by a combination of physical, phenotypic, and functional characteristics, leading to varied nomenclature.

Purpose of the Study:

  • To highlight the therapeutic potential of mesenchymal stem cells (MSCs) in disease modeling and tissue repair.
  • To discuss the pharmacological significance of MSCs, including their molecular secretion, receptor expression, and genetic manipulability.
  • To emphasize the need for a clearer understanding of MSC identity, characteristics, and origin due to current limitations in research methods.

Related Experiment Videos

Main Methods:

  • Review of existing literature on mesenchymal stem cell (MSC) isolation, culture, and characterization.
  • Analysis of in vitro studies due to the low frequency and limited knowledge of cell surface markers and origin of MSCs.
  • Examination of common culture systems used for MSC identification, including CFU-F assay and bone marrow stroma analysis.

Main Results:

  • Mesenchymal stem cells (MSCs) can be used as therapeutic agents or gene delivery vehicles in disease models.
  • MSCs demonstrate potential for replacing damaged tissues like bone, cartilage, and tendons.
  • Current research relies heavily on in vitro studies, with uncertainties regarding whether isolation conditions selectively expand specific precursors or alter cell phenotypes.

Conclusions:

  • Mesenchymal stem cells (MSCs) possess significant therapeutic potential due to their plasticity and ability to differentiate into various cell types.
  • The pharmacological relevance of MSCs stems from their secreted molecules, receptor expression, and susceptibility to genetic modification.
  • A comprehensive understanding of MSC identity and characteristics is crucial, necessitating further research to address ambiguities in their identification and behavior.