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Updated: Aug 14, 2026
Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
Published on: July 16, 2013
Effect of polychlorinated biphenyls (PCBs) on basal and OT-stimulated calcium concentrations in myometrial cells in
1Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-718 Olsztyn-Kortowo, Poland.
Abstract:
We investigated the effect of PCB-77, -126 or -153 (10 or 100 ng/ml) on free intracellular calcium concentrations([Ca2+]i) in bovine myometrial cells from days 1-5 of the estrous cycle. Cells were incubated with or without PCBs for 48 h (38 degrees C, aerated atmosphere) and thereafter [Ca2+]i was measured by means of fluorescent calcium indicator Fura-2. PCBs increased basal concentrations of [Ca2+]i measured before oxytocin (OT) challenge. The increase in [Ca2+]i in cells incubated with PCBs and challenged with OT was inhibited or delayed when compared to control OT-stimulated cells (p<0.05). The applied doses of PCBs did not affect viability of myometrial cells. In conclusion, the influence of PCBs upon intracellular calcium mobilization in myometrial cells impaired the bovine uterus contractility.
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