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Microfluidic diffusive filter for apheresis (leukapheresis).

Palaniappan Sethu1, Aaron Sin, Mehmet Toner

  • 1Surgical Services and Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School and Shriners Hospital for Children, MA 02114, Boston, USA. spalania@sbi.org

Lab on a Chip
|December 24, 2005
PubMed
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This study presents a novel microfluidic device for leukocyte depletion via apheresis. The soft lithography-fabricated filter achieves over 97% white blood cell removal from whole blood.

Area of Science:

  • Biomedical Engineering
  • Microfluidics
  • Hematology

Background:

  • Apheresis separates blood components; leukapheresis removes white blood cells.
  • Current methods include centrifugation and fiber mesh filtration.
  • Therapeutic applications of apheresis are expanding.

Purpose of the Study:

  • To design, fabricate, and test a continuous flow diffusive filter for leukocyte depletion.
  • To develop a microfluidic device using soft lithography.
  • To optimize the filter design for efficient white blood cell removal.

Main Methods:

  • Fabrication of a diffusive filter using soft lithography.
  • Utilizing micro sieves based on cell size and shape differences.
  • Development of a theoretical model for optimal diffuser shape.

Related Experiment Videos

  • Testing the device's performance at a flow rate of 5 microliters/min.

    • Main Results:

    • Achieved >97% depletion of inlet leukocytes (white blood cells).
    • Isolated approximately 50% of inlet erythrocytes (red blood cells).
    • Demonstrated a passive device requiring no external manipulation.

    Conclusions:

    • The developed microfluidic device offers efficient leukocyte depletion.
    • Soft lithography provides a viable fabrication method for this apheresis technology.
    • Potential for modifications to isolate other blood components like plasma.