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A differentially methylated region of the DAZ1 gene in spermatic and somatic cells
Zuo-Xiang Li1, Xu Ma, Zhao-Hui Wang
1Department of Genetics, Peking Union Medical College, Beijing 100730, China.
Aim:
To investigate the methylation status of the deleted in azoospermia 1 (DAZ1) gene promoter region in different cell types.
Methods:
Using CpG island Searcher software, a CpG island was found in the promoter region of the DAZ1 gene. The methylation status of this region was analyzed in sperm and leukocytes by bisulfited sequencing.
Results:
The methylation status of the CpG island in the DAZ1 gene promoter region differed in leukocytes and sperm: it was methylated in leukocytes, but unmethylated in sperm.
Conclusion:
A differentially methylated region of the DAZ1 gene exists in spermatic and somatic cells, suggesting that methylation of this region may regulate DAZ1 gene expression in different tissues.
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