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Updated: Aug 14, 2026
Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors
Published on: June 7, 2018
Characterization of an antibody that can detect the Kai1/CD82 murine metastasis suppressor
Mary C Custer1, John I Risinger, Shelley Hoover
1Laboratory of Biosystems and Cancer, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA. custema@mail.nih.gov
Background:
Kai1, also known as CD82, is a member of the tetraspanin family (TM4SF). The human homolog, KAI1, is an activation antigen of T-cells and is a metastasis suppressor for prostate and other cancers. Little is known about the mouse protein because of the lack of antibody reagents.
Methods:
Peptide immunized rabbits were used to generate polyclonal antibody to Kai1. The antibody was analyzed using immunoblotting, flow cytometry, and immunohistochemistry.
Results:
This antibody specifically recognizes murine Kai1 protein, crossreacts with rat Kai1 but not with human KAI1. The normal tissue distribution of this protein in mice is shown to be similar to that of the human homolog. Interestingly, mouse prostatic epithelium showed differential expression within the lobes.
Conclusion:
This antibody, the first described that can specifically detect murine Kai1/CD82, should be very useful in addressing the mechanism of action of Kai1 in metastatic suppression.
Insights
A new antibody specifically detects murine Kai1 (CD82), a metastasis suppressor. This tool aids research into Kai1
Area of Science:
- Immunology
- Molecular Biology
- Cancer Research
Background:
- Kai1 (CD82) is a tetraspanin protein involved in T-cell activation and cancer metastasis suppression.
- Limited understanding of murine Kai1 function due to lack of specific antibodies.
Purpose of the Study:
- To develop and characterize a novel polyclonal antibody for detecting murine Kai1.
- To enable further investigation into Kai1's role in cancer metastasis.
Main Methods:
- Generation of polyclonal antibody using peptide immunization in rabbits.
- Validation via immunoblotting, flow cytometry, and immunohistochemistry.
Main Results:
- The antibody specifically recognizes murine Kai1 and cross-reacts with rat Kai1, but not human KAI1.
- Normal tissue distribution in mice mirrors that of the human homolog.
- Differential expression of mouse prostatic epithelium observed within lobes.
Conclusions:
- This is the first antibody capable of specifically detecting murine Kai1/CD82.
- The antibody is a valuable tool for studying Kai1's mechanism in metastasis suppression.
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