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Published on: January 28, 2020
Expression of CD97 and adhesion molecules on circulating leukocytes in patients undergoing coronary artery bypass
1Department of Surgical Research and Techniques, University of Pecs, Pecs, Hungary. balazs.gasz@aok.pte.hu
Insights
Cardiopulmonary bypass (CPB) significantly activates leukocytes, indicated by CD97 expression, especially in lymphocytes. Off-pump surgery shows less leukocyte activation, highlighting CD97
Area of Science:
- Immunology
- Cardiovascular Surgery
- Biomarker Research
Background:
- Leukocyte activation contributes to adverse effects after cardiopulmonary bypass (CPB).
- CD97 is a novel cell surface molecule indicating leukocyte and lymphocyte activation.
- Adhesion molecules CD11 and CD18 are also markers of leukocyte activation.
Purpose of the Study:
- To compare CD97 and adhesion molecule expression on leukocyte subsets in patients undergoing coronary surgery with or without CPB.
- To assess the role of CD97 as a marker for CPB-related inflammatory responses.
Main Methods:
- Study included 30 patients: 20 with CPB (Group A) and 10 with off-pump (OP) surgery (Group B).
- Blood samples were collected pre-surgery, during surgery, and up to 7 days post-surgery.
- Expression of CD97, CD11, and CD18 on granulocytes, monocytes, and lymphocytes was analyzed.
Main Results:
- CPB surgery led to decreased CD97 expression on granulocytes and monocytes, followed by a significant increase peaking on postoperative days 2-3.
- CD97-positive lymphocytes gradually increased post-CPB, remaining elevated for 7 days.
- OP surgery showed only moderate changes in CD97 expression across leukocyte subsets.
- Adhesion molecule expression mirrored CD97 expression patterns.
Conclusions:
- Coronary bypass with CPB induces substantial leukocyte activation compared to OP surgery.
- Lymphocytes are highly activated and involved in leukocyte sequestration post-CPB.
- CD97 is a significant indicator of the inflammatory response associated with CPB.
Objective:
Leukocyte activation is thought to be responsible for the adverse effects and postoperative complications following cardiopulmonary bypass (CPB). A novel cell surface molecule, CD97, is a sensitive marker of leukocyte and primary lymphocyte activation. The present study aimed to determine the activation of different leukocyte subsets by comparing the expression of CD97 and adhesion molecules (CD11, CD18) in patients receiving coronary surgery with or without CPB.
Methods:
30 patients were enrolled and scheduled for coronary bypass surgery under CPB (20 patients, group A) and with off-pump (OP) operation (10 patients, group B). Blood samples were taken before and during surgery, and over the following first week.
Results:
Here, we report an early decrease in CD97 expression of granulocytes (PMN) and monocytes (MC) followed by an intensive increase reaching the maximum on postoperative days 2 and 3 in patients operated with CPB. The rate of active CD97-positive lymphocytes showed a marked, gradual increase until postoperative day 3 and remained elevated up to day 7 after CPB. OP surgery resulted in moderate alteration in the presence of CD97 on PMN, MC and lymphocytes. The expression of adhesion molecules was similar to CD97 in all leukocyte subsets.
Conclusion:
The findings about CD97 expression suggest considerable leukocyte activation following coronary bypass with CPB compared to OP surgery. The collected data show that the lymphocytes are highly activated and involved in leukocyte sequestration after CPB. Moreover, the importance of CD97 in CPB-related inflammatory response can be stated.
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