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Related Experiment Videos

Cecal ligation and puncture.

William J Hubbard1, Mashkoor Choudhry, Martin G Schwacha

  • 1Department of Surgery and Center for Surgical Research, University of Alabama, Birmingham, AL 35294-0019, USA.

Shock (Augusta, Ga.)
|December 24, 2005
PubMed
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The cecal ligation and puncture (CLP) rodent model effectively simulates sepsis and septic shock, offering a versatile platform for studying various aspects of this critical condition. Its adaptability and cost-effectiveness make it invaluable for sepsis research.

Area of Science:

  • Sepsis and Septic Shock Research
  • Animal Models in Medicine
  • Infectious Disease Pathophysiology

Background:

  • The cecal ligation and puncture (CLP) model is a widely adopted rodent method for studying sepsis and septic shock.
  • This model recapitulates key pathophysiological changes, including hyperdynamic and hypermetabolic states, progressing to hypodynamic stages and mortality.
  • Early positive blood cultures for enteric organisms are characteristic of the CLP model.

Purpose of the Study:

  • To review the utility and applications of the cecal ligation and puncture (CLP) rodent model in sepsis research.
  • To highlight the versatility and technical aspects of the CLP model for investigating various facets of sepsis.
  • To discuss strategies for overcoming challenges, such as small blood volumes, in rodent sepsis studies.

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Main Methods:

  • The cecal ligation and puncture (CLP) procedure in rodents.
  • Analysis of physiological and metabolic changes post-CLP.
  • Adaptation of micromethods or use of serial sampling for analyzing small blood volumes.

Main Results:

  • The CLP model induces a dynamic physiological state mimicking human sepsis, characterized by early bacterial dissemination.
  • Over 26 years, the CLP model has proven highly adaptable for diverse research objectives, including energetics, metabolism, and immune responses.
  • The model is cost-effective and technically straightforward, with established methods to manage small sample volumes.

Conclusions:

  • The cecal ligation and puncture (CLP) model remains a cornerstone in sepsis and septic shock research due to its reproducibility and versatility.
  • It facilitates comprehensive investigation into sepsis pathophysiology, treatment responses, and immune modulation.
  • Despite challenges with rodent blood volumes, established techniques ensure the model's continued efficacy for detailed analysis.