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Updated: Aug 14, 2026

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation
Published on: January 28, 2021
[Influence of proteinuria on the lipoprotein (a) metabolism disorder]
Dejan Petrović1, Radmila Obrenović, Biljana Stojimirović
1Klinicko-bolnicki centar "Kragujevac", Klinika za urologiju i nefrologiju, Kragujevac. aca96@EUnet.yu
Background/Aim:
Proteinuria causes lipid metabolism abnormalities. The aim of the present study was to examine the influence of proteinuria on the lipoprotein (a) metabolism disorder.
Methods:
The study included 60 patients of the male-famele ratio (M: F = 32 : 28), mean age 37.15 +/- 9.85 years with, the average endogenous creatinine clearance 86.27 +/- 19.81 ml/min, and the average body mass index (BMI) 24.18 +/- 2.23 kg/m2. Regarding the level of glomerular proteinuria, the patients were divided into four groups. The first (control) group, with proteinuria levels less than 0.25 g/24h, included 15 patients (M: F = 6 : 9), mean age 34.66 +/- 4.82 years, the mean clearance of endogenous creatinine 99.70 +/- 12.94 ml/min, and mean BMI 23.28 +/- 3.50 kg/m2. The second group, with proteinuria between 0.25 and 1.0 g/24 h, includ 15 patients (M: F = 9 : 6) with primary glomerulonephritis, mean age 37.87 +/- 9.65 years, the mean clearance of endogenous creatinine 82.85 +/- 18.48 ml/min, and mean BMI 23.83 +/- 1.57 kg/m2. The third group includ 15 patients (M : F = 8 : 7) with primary glomerulonephritis, with proteinuria between 1.0 and 3.0 g/24 h, mean age 35.67 +/- 13.29 years, the mean clearance of endogenous creatinine 82.85 +/- 18.48 ml/min, and mean BMI 23.83 +/- 1.57 kg/m2. The fourth group, with proteinuria higher than 3.0 g/24 h, included 15 patients (M: F = 9 : 6) with primary glomerulonephritis, mean age 40.40 +/- 9.75 years, the mean clearance of endogenous creatinine 80.16 +/- 20.80 ml/min, and mean BMI 24.83 +/- 1.44 kg/m2. In order to assess the influence of proteinuria on the lipoprotein (a) metabolism abnormalities we investigated 24-hour proteinuria, the colloid osmotic pressure (COP) of plasma, and the serum concentration of lipoprotein (a). The results were statistically analyzed using ANOVA, Kruscal-Wallis test, Mann-Whitney U test, chi2 test and Spearman test.
Results:
Statistically, the patients with proteinuria over 3.0 g/24 h had the significantly higher values of lipoprotein (a) in serum as compared to the control group, and the patients with proteinuria about 0.25-1.0 g/24 h. The patients with proteinuria between 1.0-3.0 g/24 h had the statistically significantly higher values of lipoprotein (a) in serum than the control group (proteinuria < 0.25 g/24 h). There was a highly statistically significant negative correlation between serum albumin concentration, COP and the concentration of lipoprotein (a) in serum. There was a highly statistically significant positive correlation between 24-hour proteinuria and the concentration of lipoprotein (a) in serum.
Conclusion:
Proteinuria leads to the deterioration of lipoprotein (a) abnormalities.
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