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A single mode fibre-optic evanescent wave biosensor.

E E Carlyon1, C R Lowe, D Reid

  • 1Institute of Biotechnology, University of Cambridge, UK.

Biosensors & Bioelectronics
|January 1, 1992
PubMed
Summary
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This study developed a single-mode fiber-optic biosensor for detecting dye concentrations and enzyme activity. The biosensor successfully monitored Penicillin G using an immobilized enzyme and a colorimetric assay.

Area of Science:

  • Optoelectronics
  • Biomedical Engineering
  • Chemical Sensing

Background:

  • Fiber-optic sensors offer sensitive detection methods.
  • Evanescent wave technology enables non-destructive analysis.
  • Developing practical biosensors is crucial for diagnostics.

Purpose of the Study:

  • To report experimental developments in constructing and operating a single-mode fiber-optic evanescent wave biosensor.
  • To demonstrate the biosensor's capability in monitoring dye concentrations and enzyme activity.
  • To validate the biosensor's practicality using a colorimetric enzyme assay.

Main Methods:

  • Utilized a single-mode silica fiber with an exposed core embedded in a silica block.
  • Employed evanescent wave interactions for sensing.

Related Experiment Videos

  • Immobilized Bacillus cereus penicillinase on the fiber core for enzyme detection.
  • Monitored the decoloration of a starch-iodine reagent at 633 nm.

    • Main Results:

    • Successfully monitored the concentration of Procion Blue MX-G dye in overlayers with varying refractive indices.
    • Demonstrated the biosensor's ability to detect Penicillin G in the 0-0.4 mM range.
    • Confirmed the colorimetric reaction's correlation with Penicillin G concentration.

    Conclusions:

    • The developed single-mode fiber-optic evanescent wave biosensor is practical for quantitative analysis.
    • The biosensor shows potential for applications in biochemical assays and diagnostics.
    • Further development could expand its utility in various sensing applications.