Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Combined inter-observer and inter-method variation in bone histomorphometry.

C D Wright1, S Vedi, N J Garrahan

  • 1Department of Medicine, University of Cambridge Clinical School, Addenbrookes Hospital, U.K.

Bone
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Gestational choline supplementation ameliorates T-maze reversal learning deficits induced by first trimester binge alcohol exposure in weanling lambs.

Alcohol (Fayetteville, N.Y.)·2024
Same author

Design and development of microformulations for rapid release of small molecules and oligonucleotides.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2023
Same author

Genome-wide Scan of Dental Fear and Anxiety Nominates Novel Genes.

Journal of dental research·2022
Same author

Publisher Correction: Parallel convolutional processing using an integrated photonic tensor core.

Nature·2021
Same author

Parallel convolutional processing using an integrated photonic tensor core.

Nature·2021
Same author

A new forest dwelling button spider from South Africa (Araneae, Theridiidae, Latrodectus).

Zootaxa·2020
Same journal

Baseline β-CTX and BMI predict suitability for deferred zoledronic acid redosing beyond 12 months in postmenopausal Indian women with osteoporosis.

Bone·2026
Same journal

Bone density-based maturation of the midpalatal suture in children aged 8-15 years.

Bone·2026
Same journal

Disrupted phosphate metabolism and SIBLING/ASARM peptide accumulation underlie impaired bone mineralization in klotho-deficient (kl/kl) mice.

Bone·2026
Same journal

Linking genetic variants to bone microstructure: Histological signatures of osteogenesis imperfecta subtypes.

Bone·2026
Same journal

The impact of alcohol consumption on bone mineral density: Insights from cross-sectional and Mendelian randomization studies.

Bone·2026
Same journal

Systemic and local predictors of medication-related osteonecrosis of the jaw in patients receiving antiresorptive therapy: The Shizuoka Kokuho Database study.

Bone·2026
See all related articles

Comparing bone biopsy measurements from manual and semi-automated techniques reveals significant differences, particularly in osteoid perimeter and width. Researchers must exercise caution when comparing bone histomorphometry data across different methods.

Area of Science:

  • Bone histomorphometry
  • Osteoporosis research
  • Biomedical engineering

Background:

  • Manual bone biopsy measurement methods are being replaced by semi-automated techniques.
  • Histomorphometrists often combine data from different observers and methods, introducing combined variation.
  • Understanding the sources of variation is crucial for accurate bone analysis.

Purpose of the Study:

  • To examine the combined effect of inter-method and inter-observer variation on bone biopsy measurements.
  • To quantify the contribution of inter-method and inter-observer variation to measurement error.
  • To assess the impact of these variations on bone area, osteoid perimeter, and osteoid width.

Main Methods:

  • Comparison of manual grid system versus semi-automated technique for iliac crest biopsy measurements.

Related Experiment Videos

  • Independent determination of inter-observer and inter-method variation.
  • Calculation of the proportion of each variation type as a percentage of combined error.

    • Main Results:

    • Combined inter-method and inter-observer variation significantly impacts osteoid perimeter measurements.
    • Inter-method variation is the primary driver of differences in osteoid width.
    • Bone area variations are mainly attributable to inherent sampling variability.

    Conclusions:

    • Caution is advised when comparing bone histomorphometry control data from different sources and techniques.
    • Inter-method and inter-observer variations introduce significant discrepancies in bone biopsy analysis.
    • Accurate interpretation of bone biopsy results requires awareness of measurement technique limitations.