Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calcium in osteoblast-enriched bone cells.

K Imai1, M W Neuman, T Kawase

  • 1Department of Oral Biochemistry, Kanagawa Dental College, Yokosuka, Japan.

Bone
|January 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular cloning of a novel protein-tyrosine phosphatase SH-PTP3 with sequence similarity to the src-homology region 2.

FEBS letters·1992
Same author

Cloning and characterization of a human cDNA encoding a novel putative cytoplasmic protein-tyrosine-phosphatase.

Biochemical and biophysical research communications·1992
Same author

Comparison of various assay systems for prostate-specific antigen standardization.

Japanese journal of clinical oncology·1992
Same author

The ubiquitous transactivator Zfp-38 is upregulated during spermatogenesis with differential transcription.

Mechanisms of development·1992
Same author

Determination of femtomole concentrations of catecholamines by high-performance liquid chromatography with peroxyoxalate chemiluminescence detection.

The Analyst·1992
Same author

Increased circulating intercellular adhesion molecule-1 in adult T cell leukemia patients.

International journal of hematology·1992

Osteoblasts near bone formation sites process more calcium, with varying exchange rates and locations within the cell. This intracellular calcium is dynamic and influenced by 1,25(OH)2D3 and calcium availability.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Bone Physiology

Background:

  • Osteoblasts are crucial for bone formation and mineralization.
  • Calcium homeostasis within bone cells is vital for their function.
  • Understanding calcium dynamics in osteoblasts informs bone health research.

Purpose of the Study:

  • To investigate the exchangeability, location, and quantity of calcium in osteoblasts.
  • To correlate calcium content and exchange with osteoblastic activity and proximity to bone formation.
  • To explore the influence of treatments on intracellular calcium dynamics.

Main Methods:

  • Isolation of neonatal rat calvarial cells via collagenase digestion.
  • Incubation with 45Ca2+ before and after various treatments.

Related Experiment Videos

  • Quantification of 45Ca2+, 40Ca2+, and DNA in cell samples; long-term cell cultures.

    • Main Results:

    • Osteoblasts near forming bone exhibit higher alkaline phosphatase activity and calcium content (17 mM/l cell water) compared to soft tissue cells (2-3 mM/l).
    • Approximately 50% of cellular calcium exchanges readily (cell membrane), 35% exchanges slowly (intracellular, mainly mitochondria), and 15% is nonexchangeable.
    • Intracellular calcium is rapidly modulated by calcium supply and influenced by 1,25(OH)2D3, which increases calcium during low supply and releases it during high supply.

    Conclusions:

    • Osteoblasts actively process and compartmentalize calcium, with distinct exchange rates and locations.
    • Proximity to mineralization sites correlates with increased osteoblast calcium processing.
    • Further research is needed to determine the directionality of calcium flux (blood-to-bone vs. bone-to-blood).