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Cloning and sequencing of the aldehyde oxidase gene from Methylobacillus sp. KY4400
Akinori Yasuhara1, Miho Akiba-Goto, Kazuo Aisaka
1BioFrontier Laboratories, Kyowa Hakko Kogyo Co., Ltd., Tokyo, Japan.
Bioscience, Biotechnology, and Biochemistry
|December 27, 2005
Abstract:
The aldehyde oxidase genes (aods) from Methylobacillus sp. KY4400 were cloned, and sequenced. The sequences for small (aodS, 489 bp), medium (aodM, 993 bp), and large (aodL, 2,328 bp) subunit genes were determined. At least one additional ORF was indispensable for the expression of enzyme activity. The structural genes contained two [2Fe-2S] centers, an FAD binding site, and a molybdenum cofactor binding site.
