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pHi controls cytoplasmic calcium in rat parotid cells.
K W Snowdowne1, B Way, G Thomas
1Department of Microbiology, School of Dentistry, University of the Pacific, San Francisco, CA 94115.
Biochimica Et Biophysica Acta
|July 27, 1992
Summary
Cytoplasmic pH (pHi) directly influences intracellular calcium ion concentration (Cai) in rat parotid cells. Manipulating pHi levels altered Cai, suggesting a tight link between these cellular processes.
Area of Science:
- Cellular Physiology
- Ion Transport
Background:
- Cytoplasmic pH (pHi) and intracellular calcium ion concentration (Cai) are critical cellular parameters.
- The relationship between pHi and Cai in rat parotid cells requires further elucidation.
Purpose of the Study:
- To investigate the modulatory effect of cytoplasmic pH (pHi) on basal intracellular calcium ion concentration (Cai) in rat parotid cells.
- To determine the direct relationship between pHi and Cai.
Main Methods:
- Utilized BCECF to measure pHi and the calcium photoprotein aequorin to measure Cai.
- Employed experimental manipulations such as NH4Cl exposure, altered CO2 partial pressure, and nigericin to modify pHi.
- Adjusted extracellular conditions, including [NaHCO3] and NH4 removal, to influence Cai.
Main Results:
- Experimental conditions that elevated pHi consistently increased Cai.
- Experimental conditions that reduced Cai also led to a decrease in Cai.
- Demonstrated a direct positive correlation between pHi and Cai in parotid cells.
Conclusions:
- Cytoplasmic pH (pHi) and intracellular calcium ion concentration (Cai) are directly interrelated in rat parotid cells.
- The regulation of Cai and pHi are tightly coupled cellular mechanisms.