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Structural consensus of RNA templates replicated by Q beta replicase.

L A Voronin1

  • 1Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.

Biochimie
|May 1, 1992
PubMed
Summary

Q beta replicase enzyme synthesizes small RNAs and phage genomic RNA. Sequence analysis reveals a conserved secondary structure in these RNAs, similar to tRNA, aiding replication.

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Area of Science:

  • Molecular Biology
  • Virology
  • RNA Structure

Background:

  • Q beta replicase is known to replicate phage genomic RNA.
  • The enzyme also synthesizes various enzyme-specific small RNAs.
  • Understanding the structure of these RNAs is crucial for comprehending replication mechanisms.

Purpose of the Study:

  • To investigate the potential for a consensus secondary structure in Q beta replicase-synthesized RNAs.
  • To compare this structure with known RNA structures like tRNA.
  • To determine if phage genomic RNA shares this structural element.

Main Methods:

  • Sequence analysis of replicating RNA molecules.
  • Secondary structure prediction based on sequence complementarity.
  • Comparative analysis with transfer RNA (tRNA) structure.

Main Results:

  • All enzyme-specific small RNAs and phage genomic RNA share a consensus secondary structure element.
  • This structure features a stalk formed by terminal complementary sequences (5'-GGG...CCCA-3').
  • Adjacent hairpins may stack with the stalk, resembling a tRNA structure.

Conclusions:

  • Q beta replicase-synthesized RNAs possess a conserved, tRNA-like secondary structure.
  • This structural element likely plays a role in RNA replication.
  • Genomic RNA of Q beta and related phages also exhibits this conserved structure.

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