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Related Experiment Videos

[Functioning of latrotoxin channels during pH changes].

E A Petrushenko, L G Storchak, N G Gimmel'reĭkh

    Biokhimiia (Moscow, Russia)
    |May 1, 1992
    PubMed
    Summary
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    Calcium (Ca2+) transport via alpha-latrotoxin channels in synaptosomes is inhibited by external pH decrease. Intracellular pH changes within 0.3 units do not affect Ca2+ influx through these channels.

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Cell Biology

    Context:

    • Synaptosomes are crucial for neurotransmission, and their function is modulated by ion fluxes.
    • Alpha-latrotoxin (LTX) forms channels in neuronal membranes, influencing calcium (Ca2+) permeability.
    • Understanding ion transport mechanisms is vital for deciphering synaptic function and dysfunction.

    Purpose:

    • To investigate the influence of pH on Ca2+ transport through LTX-formed channels in synaptosomes.
    • To determine the relationship between extracellular and intracellular pH in regulating Ca2+ influx.
    • To elucidate the role of proton gradients in LTX channel activity.

    Summary:

    • This study utilized the fluorescent probe BCECF to examine Ca2+ transport across LTX-formed channels in synaptosomes under varying pH conditions.

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  • Intracellular pH (pHi) was found to be 7.16 ± 0.09; minor intracellular pH shifts (0.1-0.3 units) did not impact Ca2+ influx.
  • A decrease in extracellular pH to 6.0 inhibited Ca2+ fluxes, while dissipation of the proton gradient affected LTX channel formation.
  • Ca2+ influx led to cytoplasmic alkalinization, mirroring the external pH, an effect independent of Na+ but blocked by cadmium.

    • Impact:

    • Reveals pH-dependent regulation of Ca2+ influx through LTX channels, impacting synaptic transmission.
    • Highlights the role of extracellular pH in modulating neuronal excitability via LTX channels.
    • Provides insights into the biophysical properties of LTX channels and their interaction with ion gradients.