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[Cytochemical ultrastructural study of adenosine 5'monophosphate hydrolysis in human spermatozoa]
B Caporiccio1, M Chalet, O Auziech
1Laboratoire de Biologie du Développement et de la Reproduction Humaine, Faculté de Médecine, Montpellier, France.
Bulletin De L'Association Des Anatomistes
|March 1, 1992
Abstract:
A specific 5'nucleotidase activity (pH 7.4) is localized by cytochemical technics, in the head (nuclear membrane, periacrosomal cytoplasm) and in the mitochondrial membrane of human spermatozoa. The effect of different effectors (manganese, nickel, fluoride, tartrate, tetramisole), studied in the head by rate assessment of cells with or without hydrolysis sites, changes the cellular distribution, the depth of reactions and their localization. The 5'nucleotidase interferences with some unspecific phosphatases are discussed.