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Updated: Aug 14, 2026

Real-Time Polymerase Chain Reaction-Based Detection and Quantification of Hepatitis B Virus DNA
Published on: December 15, 2023
[Epidemiology of hepatitis B and C in Croatia]
1Zavod za javno zdravstvo grada Zagreba, Mirogojska cesta 16, 10000 Zagreb, Hrvatska vladimira.lesnikar@zg.htnet.hr
Insights
Hepatitis B (HBV) and Hepatitis C (HCV) infections are difficult to track due to asymptomatic cases. Croatia has low HBsAg carrier rates, with infections primarily spreading among young adults through sexual contact or skin exposure.
Area of Science:
- Hepatology and Virology
- Infectious Disease Epidemiology
- Public Health Surveillance
Context:
- Hepatitis B virus (HBV) and Hepatitis C virus (HCV) infections present diagnostic challenges due to a significant number of asymptomatic cases.
- Croatia reports approximately 200 new HBV infections and HBsAg carriers annually, with a general population HBsAg carrier rate below 2%.
- HCV infection markers (anti-HCV) prevalence exceeds 1% in Croatia, with around 200 reported cases yearly, predominantly in the 20-40 age group.
Purpose:
- To determine the incidence and prevalence of Hepatitis B and C infections in Croatia.
- To identify high-risk populations and transmission routes for HBV and HCV.
- To inform public health strategies for infection control and prevention.
Summary:
- HBV predominantly affects adolescents and young adults via sexual or percutaneous routes, with specific high-risk groups identified (e.g., IV drug users, newborns of infected mothers).
- HCV incidence is highest in the 20-40 age group, primarily linked to intravenous drug use and historical blood transfusions before screening.
- Data from the Croatian Institute of Public Health indicates consistent annual infection numbers for both HBV and HCV, highlighting ongoing transmission.
Impact:
- Understanding transmission dynamics is crucial for targeted prevention programs in Croatia.
- Highlights the need for continued vigilance and screening, especially in identified high-risk populations.
- Provides baseline data for evaluating the effectiveness of public health interventions against viral hepatitis.
Abstract:
The incidence of HBV and HCV infection is hard to determine because of the high number of asymptomatic infections. According to data of the Croatian Institute of Public Health, there are 200 newly infected persons with hepatitis B and approximately the same number of newly identified HBsAg carriers occur each year. Accordingly, Croatia is among the countries with less than 2% of HBsAg carriers in the general population. In these circumstances, HBV infection is most often spread among adolescents and younger adults. The route of transmission is most often sexual (semen) or through the skin in high-risk groups. An increased risk of infection is found in newborns of HBsAg positive mothers, i.v. addicts, promiscuous individuals, male homosexuals, person in close contact with acutely ill or chronic HBsAg carriers, persons that come in contact with blood and other potentially contaminated body fluids, dialysis patients, patients with multiple blood transfusions, patients with transplanted organ or tissue, patients treated for hematologic malignancies and hemophilia, and persons who undergo acupuncture, tattooing or piercing, or travel to areas with a high prevalence of HBV infection. The estimated prevalence of HCV infection marker (anti-HCV) in the Croatian general population is more than 1% and the number of yearly infected with hepatitis C reported to the Croatian Institute of Public Health is around 200 cases. The highest incidence is found in the 20-40 age groups at a high risk of infection by the use of drug injection. At risk are persons who received transfusion of blood or blood products prior to the availability of blood screening of voluntary blood donors.
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