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Updated: Aug 14, 2026

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Extraction and Analysis of Taiwanese Green Propolis
Published on: January 7, 2019
[Study on HPLC fingerprint and quality control of Propolis]
En-Yuan Zhu1, Yu-Ling Dou, Dong-Zhi Wei
1Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Abstract
Objective:
To study on HPLC fingerprint of Propolis and to control it's quality.
Method:
The chromatographic fingerprints of seven samples from different producing areas were determined by RP-HPLC.
Result:
The chromatograms of Propolis from different producing areas were very similar.
Conclusion:
The quality of Propolis can be controlled by determination the HPLC fingerprint.
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High-Performance Liquid Chromatography: Instrumentation
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
High-Performance Liquid Chromatography: Introduction
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
