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[Studies on the changes of 5-hydroxymethyl furfuraldehyde content in Radix Rehmanniae steamed for different time]
Jun Li1, Li-Ping Zhang, Wei Liu
1Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China. lijun@hactcm.edu.cn
Objective:
To find the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Radix Rehmanniae steamed for different time.
Method:
HPLC method was used, with C18 column (6 mm x 200 mm, 5 microm), a mixture of methanol-water (15: 85) as the mobile phase. The flow rate was 1.0 mL x min(-1). The UV detection was setat 284 nm and the column temperature was set at 24 degrees C.
Result:
The linear range was from 0.01 to 0.08 microg, r = 0.999 9 (n = 5). The average recovery was 100.2% and RSD was 2.7% (n = 5).
Conclusion:
The 5-HMF content increases as the processing time prolongs in a certain time rang.
