A highly efficient transformation protocol for Micro-Tom, a model cultivar for tomato functional genomics

Area of Science:

• Plant Biotechnology
• Genetics
• Agricultural Science

Background:

• Micro-Tom is a miniature dwarf tomato cultivar widely used as a model for tomato functional genomics.
• Efficient genetic transformation methods are crucial for advancing functional genomics studies in important crop species like tomato.

Purpose of the Study:

• To establish a highly efficient protocol for Agrobacterium-mediated genetic transformation of the Micro-Tom tomato cultivar.
• To facilitate functional genomics research in tomato through improved transformation efficiency.

Main Methods:

• Agrobacterium tumefaciens (Rhizobium radiobacter) C58C1Rif(R) harboring the binary vector pIG121Hm was used to inoculate cotyledon explants of Micro-Tom.
• Chimeric adventitious buds were generated and subjected to repeated shoot elongation on selection media.
• Transgenic plants were selected and regenerated from independent transformation events.

Main Results:

• The protocol achieved a high transformation efficiency, exceeding 40% of the explants.
• Multiple transgenic plants were successfully produced from independent transformation events.
• The method generated a mass of chimeric non-transgenic and transgenic adventitious buds.

Conclusions:

• This protocol provides a powerful and efficient tool for the genetic transformation of Micro-Tom tomatoes.
• The developed method is expected to significantly accelerate functional genomics research in tomato.
• The high efficiency reported can aid in the rapid generation of transgenic lines for various studies.