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The REFOLD database: a tool for the optimization of protein expression and refolding
Michelle K M Chow1, Abdullah A Amin, Kate F Fulton
1Department of Biochemistry and Molecular Biology, PO Box 13D, Monash University, Victoria 3800, Australia.
Nucleic Acids Research
|December 31, 2005
Summary
Researchers developed REFOLD, a database of protein refolding methods to optimize renaturation of recombinant proteins expressed in Escherichia coli. This tool aids in overcoming challenges in protein analysis by providing searchable refolding protocols.
Area of Science:
- Biochemistry
- Molecular Biology
- Bioinformatics
Background:
- Proteins expressed in Escherichia coli often form insoluble inclusion bodies.
- Protein refolding is crucial for functional analysis but identifying optimal conditions is challenging.
Purpose of the Study:
- To develop a web-accessible database, REFOLD, to streamline the optimization of recombinant protein refolding.
- To provide a searchable repository of published protein refolding methods.
Main Methods:
- Compilation of published protein refolding protocols.
- Development of a web-accessible relational database (REFOLD).
- Annotation of entries for multi-parameter searching.
Main Results:
- REFOLD database currently contains over 300 annotated entries.
- The database facilitates searching of refolding methods via multiple parameters.
Conclusions:
- REFOLD serves as a valuable tool for optimizing protein renaturation processes.
- The database is expected to grow and become a comprehensive resource for researchers.