Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The TIGR Maize Database.

Agnes P Chan1, Geo Pertea, Foo Cheung

  • 1The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.

Nucleic Acids Research
|December 31, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

BDNF-DT and BDNF-AS-DT: novel genes in the BDNF locus.

Molecular psychiatry·2026
Same author

Genomic, Transcriptomic, and Regulomic Analyses Do Not Support Profound Autism as a Distinct Biological Category.

bioRxiv : the preprint server for biology·2026
Same author

Deploying a JupyterHub Server for Academic Research Using Netbooks as an Example.

Current protocols·2026
Same author

Leveraging Artificial Intelligence in Allergy, Asthma, and Immunology With Environmental Exposures.

Allergy·2026
Same author

Gene regulatory network analysis identifies dysregulation of hypoxia pathways as contributing to glioblastoma treatment resistance in females.

Biology of sex differences·2026
Same author

StringTie3 improves total RNA-seq assembly by resolving nascent and mature transcripts.

Nature methods·2026
Same journal

Correction to 'New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress'.

Nucleic acids research·2026
Same journal

VeloRM: disentangling pre- and post-splicing RNA modification dynamics at single-cell resolution.

Nucleic acids research·2026
Same journal

Accessibility of telomeric overhangs to stabilizing small-molecule ligands.

Nucleic acids research·2026
Same journal

Multivalent interactions mediate SNAIL transcription factor stimulation of the nucleosome deacetylase activity of the CoREST complex.

Nucleic acids research·2026
Same journal

Genome-wide mapping of DNA G-quadruplexes in Trypanosoma brucei chromatin reveals enrichment in coding regions and transcription start sites.

Nucleic acids research·2026
Same journal

Correction to 'The Gene Ontology knowledgebase in 2026'.

Nucleic acids research·2026
See all related articles

Researchers developed gene-enriched genomic libraries for maize, accelerating gene discovery. The TIGR Maize Database now provides public access to these assembled Zea mays sequences and annotations.

Area of Science:

  • Plant Genetics
  • Genomics
  • Bioinformatics

Background:

  • Maize is a vital crop and a key model organism for plant genetics research.
  • The large and repetitive nature of the maize genome presents challenges for gene discovery and analysis.

Purpose of the Study:

  • To accelerate gene discovery and genome analysis in maize.
  • To create a publicly accessible repository of maize genomic sequences.

Main Methods:

  • Utilized methylation filtration and high C(0)t selection to create gene-enriched genomic libraries.
  • Generated and clustered approximately 900,000 gene-enriched reads into Assembled Zea mays (AZM) sequences.
  • Assembled, annotated AZMs, and anchored them to maize chromosomes using sequenced markers.

Related Experiment Videos

Main Results:

  • Developed the TIGR Maize Database, a public repository for maize genomic sequences.
  • The current AZM release comprises 298 Mb of sequence data, including 243,807 assemblies and singletons.
  • Constructed a maize repeat database and annotated 287 maize bacterial artificial chromosome (BAC) clones along with 172 additional public BAC clones.

Conclusions:

  • The developed gene-enrichment techniques significantly enhance genic sequence representation.
  • The TIGR Maize Database provides a valuable resource for maize genomics research.
  • Public accessibility of assembled and annotated sequences facilitates further studies in maize genetics.